Maltose Binding Protein 抗体
(1 reference)Quick Overview for Maltose Binding Protein 抗体 (ABIN3020578)
抗原
See all Maltose Binding Protein (MBP) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity purification
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免疫原
- An MBP-Tag fusion protein
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亚型
- IgG
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anti-Maltose Binding Protein (MBP) (AA 43-57) antibody
MBP 适用: E. coli WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Maltose Binding Protein (MBP) antibodyMBP 适用: Bacteria WB, IP 宿主: 小鼠 Monoclonal R29 unconjugated
anti-Maltose Binding Protein (MBP) antibodyMBP 适用: E. coli WB, ELISA 宿主: 小鼠 Monoclonal 10C12 unconjugated
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应用备注
- WB: 1:1000 1:3000
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限制
- 仅限研究用
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缓冲液
- PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze / thaw cycles
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储存条件
- -20 °C / -80 °C
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: "CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules." in: Developmental and comparative immunology, Vol. 53, Issue 2, pp. 339-48, (2016) (PubMed).
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: "CgIL17-5, an ancient inflammatory cytokine in Crassostrea gigas exhibiting the heterogeneity functions compared with vertebrate interleukin17 molecules." in: Developmental and comparative immunology, Vol. 53, Issue 2, pp. 339-48, (2016) (PubMed).
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- Maltose Binding Protein (MBP)
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别名
- Maltose Binding Protein
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背景
- Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (myelin basic protein) TAG in some expression systems.
抗原
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